Alkaline and neutral Comet assay profiles of sperm DNA.
Comet assay or single cell gel electrophoresis assay detects single or double strand breaks measured at the individual cell level. Unlike the other assays described above, the comet assay measures transient genetic damage that is not a fixed change to the DNA.
Comet Assay The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells. As such, this is one of the techniques used in the area of cancer research for the evaluation of genotoxicity and effectiveness of chemoprevention.
Comet Assay The Comet Assay, or Single Cell Gel Electrophoresis (SCGE) is an electrophoresis technique used to asses the damage to genomic DNA, applicable to a range of cell types.
Although comets are very old, the oldest comet recorded is Comet Halley. They are Chinese records of this comet dating as far back as 240 BC Sir Edmund Halley predicted in 1705 that a comet which had appeared in 1531, 1607, and also 1682 would return in 1758.
Comet Assay IV uses the parameters defined by Olive et al on the principle of DNA damage and comets are scored appropriately. With its easy-to-use functionality and enviable reputation, it is clear to see why Comet Assay IV is the market-leading comet scoring system, with approximately 500 systems used in laboratories worldwide!
Originally developed to measure variation in DNA damage and repair capacity within a population of mammalian cells, applications of the comet assay now range from human and sentinel animal biomonitoring (e.g., DNA damage in earthworms crawling through toxic waste sites) to measurement of DNA damage in specific genomic sequences.
The Comet Assay: A Versatile Tool for Assessing DNA Damage Alok Dhawan, Mahima Bajpayee and Devendra Parmar New chemicals are being added each year to the existing burden of toxic substances in the environment. This has led to increased pollution of ecosystems as well as deterioration of the air, water and soil quality.
Comet assay does not require any special apparatus or chemicals. It can usually be performed on materials found in most laboratories. Based on our experience and that of others we recommend the use of the following for reliable and reproducible comet assay results.
Comet Assay IV TM is the latest in a highly successful series of comet scoring systems originally developed by Perceptive Instruments, now a part of Instem.Comet Assay IV is the professional choice for those seeking accuracy, reproducibility and GLP compliance. Getting started with Comet Assay IV is as simple as installing the software and connecting the camera.This can be done in a few.
Comprehensive measurements and results Easily graph and report your data. Comet Assay IV combines accurate, repeatable results with powerful data processing and comprehensive presentation. Once you have obtained your results, you can easily process them using the Spreadsheet Generator, which is included free with every Comet Assay IV system.
The Comet Assay in Sperm—Assessing Genotoxins in Male Germ Cells. Adolf Baumgartner, Aftab Ali, Charmaine Normington and Diana Anderson. Buy chapter. Pages 390 - 456. Chapter 16 Genotoxic Effects in Peripheral Blood and Sperm in Humans in Healthy Individuals and Those with Disease States.
Abstract. Over the past decade, the comet assay, or single cell gel electrophoresis (SCGE) has become one of the standard methods for assessing DNA damage, with applications in genototoxicity testing, human biomonitoring and molecular epidemiology, ecotoxicology, as well as fundamental research in DNA damage and repair.
Test No. 489: In Vivo Mammalian Alkaline Comet Assay; The in vivo alkaline single cell gel electrophoresis assay, also called alkaline Comet Assay is a method measuring DNA strand breaks in eukaryotic cells. Each treated group is composed of a minimum of 5 animals of one sex (or of each sex as appropriate). A positive and a vehicle control.
